Abstract Biological processes, though often imaged and visualized in two dimensions, inherently occur in at least three-dimensional space.As time-resolved volumetric imaging becomes increasingly accessible, there emerges a necessity for tools that empower non-specialists to process and interpret intricate datasets.We introduce Toner Collection Unit ThirdPeak, an open-source tool tailored for the comprehensive analysis of two- and three-dimensional track data across various scales.
Its versatile import and export options Gastrointestinal - Hemorrhoid Relief ensure seamless integration into established workflows, while the intuitive user interface allows for swift visualization and analysis of the data.When applied to live-cell diffusion data, this software demonstrates the benefits of integrating both 2D and 3D analysis, yielding valuable insights into the understanding of biological processes.